Application of solid-phase extraction (SPE) and high-performance liquid chromatography (HPLC) and comparison of both detection techniques (DAD and FLD) to analyse nesfatin-1 in fetal bovine serum samples

Abstract In this study, we propose a simple, cost-effective, and sensitive high-performance liquid chromatography with both detection techniques such as diode-array fluorescence (HPLC-DAD-FLD) for the determination of nesfatin-1 in fetal bovine serum samples. The limit (LOD) quantification (LOQ) were set at satisfactory values range 0.22–0.35 mg mL −1 0.67–1.05 , respectively (at two different wavelengths (DAD) four (FLD)). Analyte concentrations determined average value from matrix preliminary results show that SPE procedure on Isolute Si-TsOH (SCX-3) could be used further analyses human Both technique, chromatographic analysis gradient elution mode technique are fast convenient.